Lake Osborne, Florida, USA is a 378-acre (152.9 hectares) lake that is part of a system of once natural freshwater lakes lying along the western slope of the coastal ridge in Palm Beach County just west of the Florida Intracoastal Waterway and Atlantic Ocean. It is located within the C-16 drainage basin which occupies approximately 40,031 acres of land (16,200 hectares). Five drainage canals discharge directly to Lake Osborne, and Lake Osborne discharges via the E-4 canal to the C16 and C51 canals to the Lake Worth Lagoon.