Presquile National Wildlife Refuge in the U.S. state of Virginia is one of four refuges that comprise the Eastern Virginia Rivers National Wildlife Refuge Complex. The refuge is a 1,329-acre (5.38 km²) island in the James River, located approximately 20 miles (32 km) south of Richmond. It is located in the easternmost part of Chesterfield County, northeast of Hopewell. Established to protect habitat for wintering waterfowl and other migratory birds, Presquile is an important component in the network of refuges on and around the Chesapeake Bay, the largest estuary in the United States. Presquile historically provided important habitat for wintering Canada geese that breed along James Bay in eastern Canada. The refuge is also home to nesting and roosting bald eagles.