Wootton Brook is a tributary of the River Nene which runs through Northamptonshire. The brook begins south of Great Houghton flowing south west through Preston Deanery and then east in the valley between Collingtree, Grange Park and East Hunsbury. It then flows south of West Hunsbury joining the River Nene at the west side of Northampton at Upton Mill. A number of small streams join the Brook along the way.