Little Ouseburn is a small village and civil parish in the Harrogate district of North Yorkshire, England. It is situated near the A1 road motorway and 6 miles south-east of Boroughbridge. It consists of two roads, Main Street which is the residential area and Church Lane which contains a Holy Trinity Church that is listed as a grade I listed building. It also has a small brick bridge with a little stream flowing under it leading to new road which leads to Great Ouseburn.