The Woodbourne Forest and Wildlife Preserve is a protected area managed by The Nature Conservancy covering 648 acres (262 ha) in northeastern Pennsylvania. The preserve contains old fields, meadows, creeks, bogs, and forests that are home to a wide variety of animals. These include over 180 species of birds such as Pileated Woodpeckers, Great Horned Owls, and Winter Wrens. The preserve's wetlands harbor frogs, snakes, and nine species of salamander, including the Spring Salamander, Northern Two-lined Salamander, and Four-toed Salamander. The preserve's forests, part of the Allegheny Highlands forests ecoregion, contain 120 acres (49 ha) of old growth northern hardwood forest that includes Eastern Hemlock, Sweet Birch, Sugar Maple, Northern Red Oak, White Ash, and American Beech.