Ambrose Lake is a lake in Algoma District, Ontario, Canada. It is about 2,400 metres (7,874 ft) long and 130 metres (427 ft) wide, and lies between Lake Superior and Highway 17 at an elevation of 417 metres (1,368 ft) about 40 kilometres (25 mi) south of the community of White River and 50 kilometres (31 mi) northwest of Wawa.