Fernandina Beach is a city in Nassau County in the state of Florida in the United States of America, on Amelia Island. It is among Florida's northernmost cities, and is one of the principal municipalities comprising Greater Jacksonville. The area was first inhabited by the Timucuan Indian tribe. Known as the "Isle of 8 Flags", it has had the flags of the following nations flown over it since 1562: France, Spain, Great Britain, Spain (again), the Patriots of Amelia Island, the Green Cross of Florida, Mexico, the Confederate States of America, and the United States.