Wolfe Island is an island at the entrance to the Saint Lawrence River in Lake Ontario near Kingston, Ontario. Wolfe Island is part of Frontenac County, Ontario and the Township of Frontenac Islands. It is the largest of the Thousand Islands. The largest community on the island is Marysville. The island was part of the traditional hunting lands of the Tyendinaga Mohawk people and the original name of the island is Ganounkouesnot ('Long Island Standing up').